Possible causes for this rapid pH change can be:
– Inappropriate carbon dioxide pressure: Increasing or decreasing the percentage of carbon dioxide in the incubator is based on the concentration of sodium bicarbonate in the medium. For concentrations of 2 to 3.7 g/L sodium bicarbonate set the 5-10% carbon dioxide.
– Tightly close the cap of cell culture flasks then screw a quarter.
– Insufficient bicarbonate buffer: Add HEPES buffer to the final concentration of 10-25 mM.
– Bacterial, fungal or yeast contamination
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